Research Results and Reports

Biosol Mix promotes a healthy balance of microbes, insuring quality soil structure, long-term plant health and plant growth. Biosol works well in mine reclamation, native grasses, landscaping, and all growing environments. Biosol Mix was developed in the same way slow release vitamins were developed by humans. Biosol Mix's slow uniform release mimics the natural growing cycle of plants making nutrients more readily available for root absorption.

Research Proven:
Biosol Mix:

There are a number of articles linked to the items below. Please click on the Research Article link you desire to bring up the associated PDF file of the published research.

Humus Growth

Ø 5 years after 1 application of Biosol mix and Biosol, the microbial biomass equaled 37 and 34 years of natural succession

For any re-establishment of vegetation it is essential that microbial activity is restored to ensure carbon and nutrient turnover. The mediator of this process is the microbial biomass. Thus if the microbial biomass levels of a reclaimed site approach those of comparable ecosystems, one important part of the reclaimed system has been re-established successfully.

We compare the microbial biomass of two revegetation trials (established in 1982, elevation 2600-2800 m above sea level) with those of an adjacent natural succession. This succession is comprised of a series of recessional moraines of different ages. Before revegetation, the soils were bare and had very low microbial biomass levels. After 5 years the mineral fertilizer equaled 9 years of natural selection, Biosol Mix equaled 37 years and Biosol equaled 34 years.

Research Article
Soil Microbiological Properties of Reclamation Sites Compared to a Natural Succession.
By H.Insam, Kananaskis Centre for Environmental Research, The University of Calgary, Calgary, Alberta, T2N 1N4 Canada

Ø Biosol is perfectly well tolerated by plants, which responded with better growth, a deeper green of the canopy, the formation of a more complex root system, and a well balanced nutritional condition.

The special organic fertilizer BIOSOL was tested with regard to its effect on potted spruces cultivated on brown earth/semi-podsol and /or virgin soil substrates. The variants compared in the trials also included untreated controls and a mineral fertilizer variant. Particularly favorable results were achieved when appling Biosol on virgin soils with a low sorptive effect, where easily soluble mineral fertilizers can cause root etchings and are often washed away quickly. According to these results, Biosol can be recommended as a fertilizer for afforestaions in high altitudes and on virgin soils; the application of Biosol is a suitable measure to be taken in order to improve the growth and general health of the plants and to lessen the stress by transplanting.

Research Article
Concerning the Application of Special Organic Fertilizers with Difficult Afforestations
By G. Glatzel and J. Fuchs
Institute for Forest Ecology of the University of Bodenkulter, Vienna Austria

Ø Application of 2% Biosol with compost resulted in a decrease of 72% -83% for the total amount of TNT and the water extractable amount of TNT.

The use of Biosol in addition of compost has successfully been demonstrated in a study in co-operation with DEC and OVAM. The use of Biosol resulted in the formation of a specific type of complex, more precisely the Meiserheimer Complex (bacteria breaks down material to the sole carbon, nitrogen and energy source) which is very stable and able to bind the TNT-molecules irreversible to the humus matrix.

Research Article
Remediation of TNT-contaminated soils by using Biosol and compost
By Siegfried D’Haene
DEME Environmental Contractors

Ø The positive effects of Biosol may have been able to reduce ph or salt concentrations in the soil solution, or improved physical stability due to the formation of stable aggregates.

Fly-ash deposits are usually difficult to reclaim due to poor chemical and physical properties. Using different kinds of composts, organic amendments, soil conditioners and mineral fertilizers, we established a reclamation trial on an ash-dump in the Helmstedt mining area. Vitality of the grasses sown, as well as the percent of plant cover were highest on the plots amended with bark compost and BIOSOL.

Research Article
Improvement of Fly-ash Reclamation with Organic, Organo-mineral and Mineral Amendments
By Heribert Insam

Ø Due to its slow release characteristics BIOSOL is considered superior to soluble inorganic fertilizers for high elevations sites with soils of low absorption capacity and high leaching potential.

BIOSOL was tested as an alternative to mineral fertilizers in field experiments designed at revitalizing Norway spruce stands affected by forest decline in Lech, Vorarlberg. Basal area increment responded positively and significantly to both mineral fertilizer and Biosol. Foliar nutrient levels increased only slightly and no major changes in soil microbial activity or mycorrhizal statue was observed. Thus fertilization with moderate amounts of fertilizer seems to be a safe method to increase tree vigour without dramatically changing site parameters.

Research Article
Use of an Organic Fertilizer in Revitalization of Protective Forests in the Austrian Alps Affected by Forest Decline
By Gerhard Glatzel, Klaus Katzensteiner, Hubert Sterba and Kurt Haselwandter

Ø Biosol and Biosol Mix fertilizers alleviate iron nutrition deficiencies of the plants when the level of available iron is low.

Ø In another trial in a vineyard it was observed that chlorosis (Iron deficiency) of grapes was reduced after fertilization with Biosol.

Iron is an essential micronutrient for plants. Deficiency of iron causes growth disorder (iron chlorosis for example). One of the reasons for iron deficiency is that the iron is fixed very strongly in the soil and is not available for the plants. Hydroxamate siderophores are able to change the structures where the iron is fixed. So the iron can be used by the plants. Hydroxamate siderophores are produced by micro-organisms (fungi or bacteria) in the soil.

In this study the researches tried to find out whether if it is possible to rise the content of hydroxamate siderophores in soil through fertilization. They tested Biosol, Bactosol, chicken dung and grape mark compost.
The results indicate that Biosol as well as Bactosol lead to remarkable higher concentrations of siderophores.

Research Article
Hydroxamate Siderophore Content of Organic Fertilizers
Dept of Microbiology, University of Innsbruck – Kurt Haselwnadter & Renate Krismer
Dept of Forestry, University of Florida – Hanspaul Holzmann & C.P. Reid

Ø Dry fungal biomass of dried mycelium (Biosol) is not only an organic fertilizer providing nutrients to crop plants and improving humus in the soil, but also a good inducer of resistance against root diseases such as Fusariem wilt. Its application may have multiple effects for both crop nutrition and crop protection. It was recently reported that dried mycelium applied to the soil protected corn plants against fusarium.

Research Article
Extracts of Killed Penicillium Chrysogenum Induce Resistance Against Fusarium Wilt of Melon
By Hezhong Dong and Yigal Cohen

Ø Dry fungal biomass of dried mycelium (Biosol) seems to provide partial control of nematodes under natural conditions. Partial control may gradually build up to almost complete control of the nematode in the following season if treatments of dry mycelium are repeated. Such a strategy may well fit in with nematode control in Organic farming.


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Greenhouse without Agro Biosol® Strong parasitic attack with nemathodes Harvest only partial possible	Greenhouse with Agro Biosol® Redced attack with nemathods Normal harvest


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Roots without Agro Biosol® Strong parasitic attack with nemathodes Strong forming of galls	Roots with Agro Biosol® Reduced attack with nemathods Low forming of galls

Ø Dry mycelium was proved by this study, as well as other studies to behave as an efficient organic fertilizer. Plant growth was enhanced several fold in treated plots compared to controls.

Research Article
Dry Mycelium of Penicillium chrysogenum Protects Cucumber and Tomato Plants against the Root-knot Nematode Meloidogyne javanica
By Dror Gotlieb, Yuji Oka, Bat-Hen Ben Daniel and Yigal Cohen

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Agro Biosol on Roses